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$(A) Schematic of [U- 13 C]glucose labeling. m+2-labeled citrate generated from pyruvate oxidation is further oxidized to m+2-labeled malate in the canonical TCA cycle. (B) Fractional m+2 enrichment of malate relative to m+2 citrate (mal+2/cit+2) from 82 NSCLC lines cultured with [U- C]glucose for 6 h, obtained from a published dataset. (C) Bubble plot of gene set enrichment analysis depicting gene sets enriched among genes positively correlating with the mal+2/cit+2 ratio in 69 NSCLC lines. (D) Simple linear regression comparing mitochondrial oxygen consumption rates (average of 6–8 replicates/cell line) and mal+2/cit+2 from [U- 13 C]glucose (average of 3 replicates/cell line). Correlation was determined using Pearson ( r ). (E) Mal+2/cit+2 from [U- 13 C]glucose in A549 cells treated with indicated concentrations of phenformin for 24 h. b.l.q, fractional enrichment below the limit of quantification. (F and G) Mal+2/cit+2 from [U- 13 C]glucose in NSCLC lines treated with vehicle or 1 μM FCCP for 24 h (F) or expressing either empty vector or <t>mito</t> Lb <t>NOX</t> (G). Data are mean ± SD with n = 3 independent replicates (E–G). Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test relative to vehicle (F) or empty vector-expressing cells (G). See also and .$
Mito Lb Nox Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mito+lb+nox+cdna/pmc13045649-418-152-156?v=Addgene+inc
Average 93 stars, based on 12 article reviews

mito lb nox cdna - by Bioz Stars, 2026-07

93/100 stars

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1) Product Images from "Citrate clearance is a major function of aconitase 2 in the canonical TCA cycle"

Article Title: Citrate clearance is a major function of aconitase 2 in the canonical TCA cycle

Journal: Cell

doi: 10.1016/j.cell.2026.01.028

$(A) Schematic of [U- 13 C]glucose labeling. m+2-labeled citrate generated from pyruvate oxidation is further oxidized to m+2-labeled malate in the canonical TCA cycle. (B) Fractional m+2 enrichment of malate relative to m+2 citrate (mal+2/cit+2) from 82 NSCLC lines cultured with [U- C]glucose for 6 h, obtained from a published dataset. (C) Bubble plot of gene set enrichment analysis depicting gene sets enriched among genes positively correlating with the mal+2/cit+2 ratio in 69 NSCLC lines. (D) Simple linear regression comparing mitochondrial oxygen consumption rates (average of 6–8 replicates/cell line) and mal+2/cit+2 from [U- 13 C]glucose (average of 3 replicates/cell line). Correlation was determined using Pearson ( r ). (E) Mal+2/cit+2 from [U- 13 C]glucose in A549 cells treated with indicated concentrations of phenformin for 24 h. b.l.q, fractional enrichment below the limit of quantification. (F and G) Mal+2/cit+2 from [U- 13 C]glucose in NSCLC lines treated with vehicle or 1 μM FCCP for 24 h (F) or expressing either empty vector or mito Lb NOX (G). Data are mean ± SD with n = 3 independent replicates (E–G). Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test relative to vehicle (F) or empty vector-expressing cells (G). See also and .$
Figure Legend Snippet: (A) Schematic of [U- 13 C]glucose labeling. m+2-labeled citrate generated from pyruvate oxidation is further oxidized to m+2-labeled malate in the canonical TCA cycle. (B) Fractional m+2 enrichment of malate relative to m+2 citrate (mal+2/cit+2) from 82 NSCLC lines cultured with [U- C]glucose for 6 h, obtained from a published dataset. (C) Bubble plot of gene set enrichment analysis depicting gene sets enriched among genes positively correlating with the mal+2/cit+2 ratio in 69 NSCLC lines. (D) Simple linear regression comparing mitochondrial oxygen consumption rates (average of 6–8 replicates/cell line) and mal+2/cit+2 from [U- 13 C]glucose (average of 3 replicates/cell line). Correlation was determined using Pearson ( r ). (E) Mal+2/cit+2 from [U- 13 C]glucose in A549 cells treated with indicated concentrations of phenformin for 24 h. b.l.q, fractional enrichment below the limit of quantification. (F and G) Mal+2/cit+2 from [U- 13 C]glucose in NSCLC lines treated with vehicle or 1 μM FCCP for 24 h (F) or expressing either empty vector or mito Lb NOX (G). Data are mean ± SD with n = 3 independent replicates (E–G). Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test relative to vehicle (F) or empty vector-expressing cells (G). See also and .

Techniques Used: Labeling, Generated, Cell Culture, Expressing, Plasmid Preparation

$(A) Schematic depicting pyruvate dehydrogenase (PDH) regulation by NAD + /NADH and PDH kinase 1 (PDK1). DCA, dichloroacetate; MPCi, mitochondrial pyruvate carrier inhibition. (B) Immunoblot of empty vector- and mito Lb NOX-expressing cells treated with vehicle or 2.5 μM phenformin for 24 h. (C) Fractional m+2 enrichment of citrate from [U- 13 C]glucose in empty vector- and mito Lb NOX-expressing Calu1 cells treated with vehicle or 2.5 μM phenformin for 24 h. (D and E) Fractional m+2 enrichment of citrate (D) and mal+2/cit+2 (E) from [U- 13 C]glucose in Calu1 cells expressing either control sgRNA (sgAAVS1) or sgRNA targeting PDK1 treated with vehicle or 2.5 μM phenformin for 24 h. b.l.q, fractional enrichment below the limit of quantification. (F) Citrate pools plotted against mal+2/cit+2 from [U- 13 C]glucose in 16 NSCLC lines, each averaged across three replicates. Correlation was determined using Pearson ( r ). (G) Metabolites ranked by Pearson correlation ( r ) comparing levels of 225 metabolites (DepMap) and mal+2/cit+2 from [U- C]glucose in 60 NSCLC lines from a published dataset. (H) Simple linear regression comparing the fold change in citrate levels and the change in mal+2/cit+2 from [U- 13 C]glucose with mito Lb NOX expression compared with empty vector (average of 3 replicates per line). Correlation was determined using Pearson ( r ). (I) Log 2 (fold change) of indicated metabolites in Calu1 PDK1 -edited cells relative to the average of control cells (sgAAVS1), shown in triplicate. (J) Mal+2/cit+2 from [U- 13 C]glucose in A549 and Calu1 cells. Data are mean ± SD with n = 3 (C, I, and J), n = 6 (D), or n = 5–6 (E) independent replicates. Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test relative to vehicle (C and J) or to sgAAVS1 control cells (D and E). See also .$
Figure Legend Snippet: (A) Schematic depicting pyruvate dehydrogenase (PDH) regulation by NAD + /NADH and PDH kinase 1 (PDK1). DCA, dichloroacetate; MPCi, mitochondrial pyruvate carrier inhibition. (B) Immunoblot of empty vector- and mito Lb NOX-expressing cells treated with vehicle or 2.5 μM phenformin for 24 h. (C) Fractional m+2 enrichment of citrate from [U- 13 C]glucose in empty vector- and mito Lb NOX-expressing Calu1 cells treated with vehicle or 2.5 μM phenformin for 24 h. (D and E) Fractional m+2 enrichment of citrate (D) and mal+2/cit+2 (E) from [U- 13 C]glucose in Calu1 cells expressing either control sgRNA (sgAAVS1) or sgRNA targeting PDK1 treated with vehicle or 2.5 μM phenformin for 24 h. b.l.q, fractional enrichment below the limit of quantification. (F) Citrate pools plotted against mal+2/cit+2 from [U- 13 C]glucose in 16 NSCLC lines, each averaged across three replicates. Correlation was determined using Pearson ( r ). (G) Metabolites ranked by Pearson correlation ( r ) comparing levels of 225 metabolites (DepMap) and mal+2/cit+2 from [U- C]glucose in 60 NSCLC lines from a published dataset. (H) Simple linear regression comparing the fold change in citrate levels and the change in mal+2/cit+2 from [U- 13 C]glucose with mito Lb NOX expression compared with empty vector (average of 3 replicates per line). Correlation was determined using Pearson ( r ). (I) Log 2 (fold change) of indicated metabolites in Calu1 PDK1 -edited cells relative to the average of control cells (sgAAVS1), shown in triplicate. (J) Mal+2/cit+2 from [U- 13 C]glucose in A549 and Calu1 cells. Data are mean ± SD with n = 3 (C, I, and J), n = 6 (D), or n = 5–6 (E) independent replicates. Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test relative to vehicle (C and J) or to sgAAVS1 control cells (D and E). See also .

Techniques Used: Inhibition, Western Blot, Plasmid Preparation, Expressing, Control

(A and B) Citrate levels (A) and population doublings (B) in cells expressing indicated sgRNA. (C and D) Population doublings (C) and citrate levels (D) of control (sgAAVS1, –) or ACO2 -edited cells (+) that additionally express either control sgRNA (sgAAVS1) or sgRNA targeting CS , treated as indicated for 72 h (C) or 24 h (D). (E and F) Schematic depicting competition experiment (left). A549 cells with sgAAVS1 expressing mCherry were mixed 1:1 with cells expressing BFP and edited with control (sgAAVS1), sgACO2, sgCS, or both sgACO2 and sgCS and assessed over time (E) or assessed after 21 days in culture with vehicle or indicated concentrations of pyruvate (F). (G) Population doublings of ACO2 -edited cells expressing empty vector (–) or SLC25A1 cDNA (+). Data n = 1 (E) or are mean ± SD with n = 3 (A–D, F, and G) independent replicates. Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test relative to sgAAVS1 control cells (A), relative to vehicle (C, D, and F) or relative to sgACO2/empty vector cells (G). See also and .

Figure Legend Snippet: (A and B) Citrate levels (A) and population doublings (B) in cells expressing indicated sgRNA. (C and D) Population doublings (C) and citrate levels (D) of control (sgAAVS1, –) or ACO2 -edited cells (+) that additionally express either control sgRNA (sgAAVS1) or sgRNA targeting CS , treated as indicated for 72 h (C) or 24 h (D). (E and F) Schematic depicting competition experiment (left). A549 cells with sgAAVS1 expressing mCherry were mixed 1:1 with cells expressing BFP and edited with control (sgAAVS1), sgACO2, sgCS, or both sgACO2 and sgCS and assessed over time (E) or assessed after 21 days in culture with vehicle or indicated concentrations of pyruvate (F). (G) Population doublings of ACO2 -edited cells expressing empty vector (–) or SLC25A1 cDNA (+). Data n = 1 (E) or are mean ± SD with n = 3 (A–D, F, and G) independent replicates. Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test relative to sgAAVS1 control cells (A), relative to vehicle (C, D, and F) or relative to sgACO2/empty vector cells (G). See also and .

Techniques Used: Expressing, Control, Plasmid Preparation

(A) Serum citrate concentration in mice provided drinking water supplemented with NaCl (control) or 3% citrate 10 days post tamoxifen administration (control water: n = 12 Aco2 +/+ , 11 Aco2 fl/fl ; citrate water: n = 14 Aco2 +/+ , 10 Aco2 fl/fl ). (B) Survival curves of indicated mice post tamoxifen (TAM) administration (control water: n = 4 Aco2 +/+ , 9 Aco2 fl/fl ; citrate water: n = 9 Aco2 +/+ , 12 Aco2 fl/fl ). (C) Violin plot depicting Z scores of 122 ISR-related genes in kidneys harvested from mice provided regular water or water supplemented with 3% citrate 10 days post tamoxifen administration ( n = 6 for all conditions except n = 7 for Aco2 fl/fl mice on 3% citrate). (D) Normalized enrichment scores of gene sets related to kidney injury or proximal tubule (PT) identity from RNA sequencing of kidneys described in (C), comparing Aco2 fl/fl vs. Aco2 +/+ animals on regular water (left column) or 3% citrate (right column). Selected gene sets represent genes increased or decreased following acute kidney injury (AKI), associated with AKI in PT cells specifically, or clusters associated with normal PT identity (segments 1–3) or injury. (E) SLC13A2 and SLC13A5 expression in cancer cell lines of kidney ( n = 51) or liver ( n = 27) lineage (TPM, transcripts per million). (F) Citrate levels in HepG2 cells cultured as indicated for 24 h. (G and H) Percent DAPI-positivity (G) or population doublings (H) in HepG2 cells cultured as indicated for 3 days. (I) Percent DAPI-positive cells of indicated genotype cultured in 5 mM (A549) or 1 mM (Calu1) citrate for 3 days. (J) Cellular competition experiment mixing ACO2 -edited cells expressing GFP (“sgACO2-GFP”) 1:1 with cells expressing SLC13A2 cDNA and either sgAAVS1 or sgACO2 and passaged with vehicle or indicated concentrations of citrate for 21 days. Shown are the percent changes in green fluorescent protein (GFP)-negative cells cultured with citrate relative to vehicle at each time point. (K) Immunoblot of cells with control (sgAAVS1; –) or ACO2 editing (+) expressing EGFP (–) or SLC13A2 cDNA (+), cultured as indicated for 24 h. Data are n = 1 (J) or mean ± SD with n = 3 (F–I) independent replicates. Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test as indicated (C), relative to control water (A), relative to vehicle (F–H), or relative to sgAAVS1 + SLC13A2 -expressing cells (I), using log-rank (Mantel-Cox) test (B), or using the fgsea package in R (D). See also .

Figure Legend Snippet: (A) Serum citrate concentration in mice provided drinking water supplemented with NaCl (control) or 3% citrate 10 days post tamoxifen administration (control water: n = 12 Aco2 +/+ , 11 Aco2 fl/fl ; citrate water: n = 14 Aco2 +/+ , 10 Aco2 fl/fl ). (B) Survival curves of indicated mice post tamoxifen (TAM) administration (control water: n = 4 Aco2 +/+ , 9 Aco2 fl/fl ; citrate water: n = 9 Aco2 +/+ , 12 Aco2 fl/fl ). (C) Violin plot depicting Z scores of 122 ISR-related genes in kidneys harvested from mice provided regular water or water supplemented with 3% citrate 10 days post tamoxifen administration ( n = 6 for all conditions except n = 7 for Aco2 fl/fl mice on 3% citrate). (D) Normalized enrichment scores of gene sets related to kidney injury or proximal tubule (PT) identity from RNA sequencing of kidneys described in (C), comparing Aco2 fl/fl vs. Aco2 +/+ animals on regular water (left column) or 3% citrate (right column). Selected gene sets represent genes increased or decreased following acute kidney injury (AKI), associated with AKI in PT cells specifically, or clusters associated with normal PT identity (segments 1–3) or injury. (E) SLC13A2 and SLC13A5 expression in cancer cell lines of kidney ( n = 51) or liver ( n = 27) lineage (TPM, transcripts per million). (F) Citrate levels in HepG2 cells cultured as indicated for 24 h. (G and H) Percent DAPI-positivity (G) or population doublings (H) in HepG2 cells cultured as indicated for 3 days. (I) Percent DAPI-positive cells of indicated genotype cultured in 5 mM (A549) or 1 mM (Calu1) citrate for 3 days. (J) Cellular competition experiment mixing ACO2 -edited cells expressing GFP (“sgACO2-GFP”) 1:1 with cells expressing SLC13A2 cDNA and either sgAAVS1 or sgACO2 and passaged with vehicle or indicated concentrations of citrate for 21 days. Shown are the percent changes in green fluorescent protein (GFP)-negative cells cultured with citrate relative to vehicle at each time point. (K) Immunoblot of cells with control (sgAAVS1; –) or ACO2 editing (+) expressing EGFP (–) or SLC13A2 cDNA (+), cultured as indicated for 24 h. Data are n = 1 (J) or mean ± SD with n = 3 (F–I) independent replicates. Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test as indicated (C), relative to control water (A), relative to vehicle (F–H), or relative to sgAAVS1 + SLC13A2 -expressing cells (I), using log-rank (Mantel-Cox) test (B), or using the fgsea package in R (D). See also .

Techniques Used: Concentration Assay, Control, RNA Sequencing, Expressing, Cell Culture, Western Blot

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mito lb nox cdna (Addgene inc)

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$(A) Schematic of [U- 13 C]glucose labeling. m+2-labeled citrate generated from pyruvate oxidation is further oxidized to m+2-labeled malate in the canonical TCA cycle. (B) Fractional m+2 enrichment of malate relative to m+2 citrate (mal+2/cit+2) from 82 NSCLC lines cultured with [U- C]glucose for 6 h, obtained from a published dataset. (C) Bubble plot of gene set enrichment analysis depicting gene sets enriched among genes positively correlating with the mal+2/cit+2 ratio in 69 NSCLC lines. (D) Simple linear regression comparing mitochondrial oxygen consumption rates (average of 6–8 replicates/cell line) and mal+2/cit+2 from [U- 13 C]glucose (average of 3 replicates/cell line). Correlation was determined using Pearson ( r ). (E) Mal+2/cit+2 from [U- 13 C]glucose in A549 cells treated with indicated concentrations of phenformin for 24 h. b.l.q, fractional enrichment below the limit of quantification. (F and G) Mal+2/cit+2 from [U- 13 C]glucose in NSCLC lines treated with vehicle or 1 μM FCCP for 24 h (F) or expressing either empty vector or <t>mito</t> Lb <t>NOX</t> (G). Data are mean ± SD with n = 3 independent replicates (E–G). Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test relative to vehicle (F) or empty vector-expressing cells (G). See also and .$
Mito Lb Nox Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lb nox mito cdnas (Twist Bioscience)

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$(A) Schematic of [U- 13 C]glucose labeling. m+2-labeled citrate generated from pyruvate oxidation is further oxidized to m+2-labeled malate in the canonical TCA cycle. (B) Fractional m+2 enrichment of malate relative to m+2 citrate (mal+2/cit+2) from 82 NSCLC lines cultured with [U- C]glucose for 6 h, obtained from a published dataset. (C) Bubble plot of gene set enrichment analysis depicting gene sets enriched among genes positively correlating with the mal+2/cit+2 ratio in 69 NSCLC lines. (D) Simple linear regression comparing mitochondrial oxygen consumption rates (average of 6–8 replicates/cell line) and mal+2/cit+2 from [U- 13 C]glucose (average of 3 replicates/cell line). Correlation was determined using Pearson ( r ). (E) Mal+2/cit+2 from [U- 13 C]glucose in A549 cells treated with indicated concentrations of phenformin for 24 h. b.l.q, fractional enrichment below the limit of quantification. (F and G) Mal+2/cit+2 from [U- 13 C]glucose in NSCLC lines treated with vehicle or 1 μM FCCP for 24 h (F) or expressing either empty vector or <t>mito</t> Lb <t>NOX</t> (G). Data are mean ± SD with n = 3 independent replicates (E–G). Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test relative to vehicle (F) or empty vector-expressing cells (G). See also and .$
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mito lb nox cdnas (Addgene inc)

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$(A) Schematic of [U- 13 C]glucose labeling. m+2-labeled citrate generated from pyruvate oxidation is further oxidized to m+2-labeled malate in the canonical TCA cycle. (B) Fractional m+2 enrichment of malate relative to m+2 citrate (mal+2/cit+2) from 82 NSCLC lines cultured with [U- C]glucose for 6 h, obtained from a published dataset. (C) Bubble plot of gene set enrichment analysis depicting gene sets enriched among genes positively correlating with the mal+2/cit+2 ratio in 69 NSCLC lines. (D) Simple linear regression comparing mitochondrial oxygen consumption rates (average of 6–8 replicates/cell line) and mal+2/cit+2 from [U- 13 C]glucose (average of 3 replicates/cell line). Correlation was determined using Pearson ( r ). (E) Mal+2/cit+2 from [U- 13 C]glucose in A549 cells treated with indicated concentrations of phenformin for 24 h. b.l.q, fractional enrichment below the limit of quantification. (F and G) Mal+2/cit+2 from [U- 13 C]glucose in NSCLC lines treated with vehicle or 1 μM FCCP for 24 h (F) or expressing either empty vector or <t>mito</t> Lb <t>NOX</t> (G). Data are mean ± SD with n = 3 independent replicates (E–G). Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test relative to vehicle (F) or empty vector-expressing cells (G). See also and .$
Mito Lb Nox Cdnas, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Journal: Cell

Article Title: Citrate clearance is a major function of aconitase 2 in the canonical TCA cycle

doi: 10.1016/j.cell.2026.01.028

Figure Lengend Snippet: (A) Schematic of [U- 13 C]glucose labeling. m+2-labeled citrate generated from pyruvate oxidation is further oxidized to m+2-labeled malate in the canonical TCA cycle. (B) Fractional m+2 enrichment of malate relative to m+2 citrate (mal+2/cit+2) from 82 NSCLC lines cultured with [U- C]glucose for 6 h, obtained from a published dataset. (C) Bubble plot of gene set enrichment analysis depicting gene sets enriched among genes positively correlating with the mal+2/cit+2 ratio in 69 NSCLC lines. (D) Simple linear regression comparing mitochondrial oxygen consumption rates (average of 6–8 replicates/cell line) and mal+2/cit+2 from [U- 13 C]glucose (average of 3 replicates/cell line). Correlation was determined using Pearson ( r ). (E) Mal+2/cit+2 from [U- 13 C]glucose in A549 cells treated with indicated concentrations of phenformin for 24 h. b.l.q, fractional enrichment below the limit of quantification. (F and G) Mal+2/cit+2 from [U- 13 C]glucose in NSCLC lines treated with vehicle or 1 μM FCCP for 24 h (F) or expressing either empty vector or mito Lb NOX (G). Data are mean ± SD with n = 3 independent replicates (E–G). Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test relative to vehicle (F) or empty vector-expressing cells (G). See also and .

Article Snippet: The following sgRNA sequences were used: Chr8: 5’ GACATTTCTTTCCCCACTGG 3’ AAVS1: 5’ GGGGCCACTAGGGACAGGAT 3’ ACO2 ( homo sapiens ) guide 1: 5’ AGCGAGGCAAGTCGTACCTG 3’ ACO2 ( homo sapiens ) guide 2: 5’ CCAGCCAGGAAATTGAGCG 3’ PDK1 ( homo sapiens ) guide 1: 5’ GAACTGCTTCATGGAGAGCG 3’ PDK1 ( homo sapiens ) guide 2: 5’ TTGCCGCAGAAACATAAATG 3’ CS ( homo sapiens ) guide 1: 5’ CAACATGGCAAGACGGTGGT 3’ CS ( homo sapiens ) guide 2: 5’ AACTGGACATATCCCAACAG 3’ ACL ( homo sapiens ) guide 1: 5’ ACCAGCTGATCAAACGTCG 3’ ACL ( homo sapiens ) guide 2: 5’ AGAATCGGTTCAAGTATGCT 3’ ACO1 ( homo sapiens ) guide 1: 5’ CCATTGGATCCTGTACAACC 3’ ACO1 ( homo sapiens ) guide 2: 5’ TCCTGCAGGATGACACGAGC 3’ SLC25A1 ( homo sapiens ): 5’ CGTCTTCACGTACTCGGTG 3’ ACO2 ( mus musculus ) guide 1: 5’ GCCAACCAGGAGATCGAGCG 3’ ACO2 ( mus musculus ) guide 2: 5’ ACTGATTCGCACACCCCCAA 3’ To stably introduce expression of mitochondrially-targeted Lactobacillus brevis NADH oxidase, mito Lb NOX cDNA (Addgene, 74448) was cloned into pCDH-CMV-MCS-EF1α-puro (System Biosciences, CD510B-1).

Techniques: Labeling, Generated, Cell Culture, Expressing, Plasmid Preparation

Journal: Cell

Article Title: Citrate clearance is a major function of aconitase 2 in the canonical TCA cycle

doi: 10.1016/j.cell.2026.01.028

Figure Lengend Snippet: (A) Schematic depicting pyruvate dehydrogenase (PDH) regulation by NAD + /NADH and PDH kinase 1 (PDK1). DCA, dichloroacetate; MPCi, mitochondrial pyruvate carrier inhibition. (B) Immunoblot of empty vector- and mito Lb NOX-expressing cells treated with vehicle or 2.5 μM phenformin for 24 h. (C) Fractional m+2 enrichment of citrate from [U- 13 C]glucose in empty vector- and mito Lb NOX-expressing Calu1 cells treated with vehicle or 2.5 μM phenformin for 24 h. (D and E) Fractional m+2 enrichment of citrate (D) and mal+2/cit+2 (E) from [U- 13 C]glucose in Calu1 cells expressing either control sgRNA (sgAAVS1) or sgRNA targeting PDK1 treated with vehicle or 2.5 μM phenformin for 24 h. b.l.q, fractional enrichment below the limit of quantification. (F) Citrate pools plotted against mal+2/cit+2 from [U- 13 C]glucose in 16 NSCLC lines, each averaged across three replicates. Correlation was determined using Pearson ( r ). (G) Metabolites ranked by Pearson correlation ( r ) comparing levels of 225 metabolites (DepMap) and mal+2/cit+2 from [U- C]glucose in 60 NSCLC lines from a published dataset. (H) Simple linear regression comparing the fold change in citrate levels and the change in mal+2/cit+2 from [U- 13 C]glucose with mito Lb NOX expression compared with empty vector (average of 3 replicates per line). Correlation was determined using Pearson ( r ). (I) Log 2 (fold change) of indicated metabolites in Calu1 PDK1 -edited cells relative to the average of control cells (sgAAVS1), shown in triplicate. (J) Mal+2/cit+2 from [U- 13 C]glucose in A549 and Calu1 cells. Data are mean ± SD with n = 3 (C, I, and J), n = 6 (D), or n = 5–6 (E) independent replicates. Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test relative to vehicle (C and J) or to sgAAVS1 control cells (D and E). See also .

Techniques: Inhibition, Western Blot, Plasmid Preparation, Expressing, Control

Journal: Cell

Article Title: Citrate clearance is a major function of aconitase 2 in the canonical TCA cycle

doi: 10.1016/j.cell.2026.01.028

Figure Lengend Snippet: (A and B) Citrate levels (A) and population doublings (B) in cells expressing indicated sgRNA. (C and D) Population doublings (C) and citrate levels (D) of control (sgAAVS1, –) or ACO2 -edited cells (+) that additionally express either control sgRNA (sgAAVS1) or sgRNA targeting CS , treated as indicated for 72 h (C) or 24 h (D). (E and F) Schematic depicting competition experiment (left). A549 cells with sgAAVS1 expressing mCherry were mixed 1:1 with cells expressing BFP and edited with control (sgAAVS1), sgACO2, sgCS, or both sgACO2 and sgCS and assessed over time (E) or assessed after 21 days in culture with vehicle or indicated concentrations of pyruvate (F). (G) Population doublings of ACO2 -edited cells expressing empty vector (–) or SLC25A1 cDNA (+). Data n = 1 (E) or are mean ± SD with n = 3 (A–D, F, and G) independent replicates. Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test relative to sgAAVS1 control cells (A), relative to vehicle (C, D, and F) or relative to sgACO2/empty vector cells (G). See also and .

Techniques: Expressing, Control, Plasmid Preparation

Journal: Cell

Article Title: Citrate clearance is a major function of aconitase 2 in the canonical TCA cycle

doi: 10.1016/j.cell.2026.01.028

Figure Lengend Snippet: (A) Serum citrate concentration in mice provided drinking water supplemented with NaCl (control) or 3% citrate 10 days post tamoxifen administration (control water: n = 12 Aco2 +/+ , 11 Aco2 fl/fl ; citrate water: n = 14 Aco2 +/+ , 10 Aco2 fl/fl ). (B) Survival curves of indicated mice post tamoxifen (TAM) administration (control water: n = 4 Aco2 +/+ , 9 Aco2 fl/fl ; citrate water: n = 9 Aco2 +/+ , 12 Aco2 fl/fl ). (C) Violin plot depicting Z scores of 122 ISR-related genes in kidneys harvested from mice provided regular water or water supplemented with 3% citrate 10 days post tamoxifen administration ( n = 6 for all conditions except n = 7 for Aco2 fl/fl mice on 3% citrate). (D) Normalized enrichment scores of gene sets related to kidney injury or proximal tubule (PT) identity from RNA sequencing of kidneys described in (C), comparing Aco2 fl/fl vs. Aco2 +/+ animals on regular water (left column) or 3% citrate (right column). Selected gene sets represent genes increased or decreased following acute kidney injury (AKI), associated with AKI in PT cells specifically, or clusters associated with normal PT identity (segments 1–3) or injury. (E) SLC13A2 and SLC13A5 expression in cancer cell lines of kidney ( n = 51) or liver ( n = 27) lineage (TPM, transcripts per million). (F) Citrate levels in HepG2 cells cultured as indicated for 24 h. (G and H) Percent DAPI-positivity (G) or population doublings (H) in HepG2 cells cultured as indicated for 3 days. (I) Percent DAPI-positive cells of indicated genotype cultured in 5 mM (A549) or 1 mM (Calu1) citrate for 3 days. (J) Cellular competition experiment mixing ACO2 -edited cells expressing GFP (“sgACO2-GFP”) 1:1 with cells expressing SLC13A2 cDNA and either sgAAVS1 or sgACO2 and passaged with vehicle or indicated concentrations of citrate for 21 days. Shown are the percent changes in green fluorescent protein (GFP)-negative cells cultured with citrate relative to vehicle at each time point. (K) Immunoblot of cells with control (sgAAVS1; –) or ACO2 editing (+) expressing EGFP (–) or SLC13A2 cDNA (+), cultured as indicated for 24 h. Data are n = 1 (J) or mean ± SD with n = 3 (F–I) independent replicates. Significance was assessed using two-way ANOVA with Sidak’s multiple-comparisons post-test as indicated (C), relative to control water (A), relative to vehicle (F–H), or relative to sgAAVS1 + SLC13A2 -expressing cells (I), using log-rank (Mantel-Cox) test (B), or using the fgsea package in R (D). See also .

Techniques: Concentration Assay, Control, RNA Sequencing, Expressing, Cell Culture, Western Blot